Comparison of the Contents of Multiple Constituents in Different Medicinal Parts of Porana racemosa / 中国药房

OBJECTIVE：To establish a method for the simultaneous determination of eight constituents ，such as scopolin ， scopoletin，chlorogenic acid ，cryptochlorogenic acid ，neochlorogenic acid ，isochlorogenic acid A ，isochlorogenic acid B ，and isochlorogenic acid C ，in different medicinal parts （stems，twigs and leaves ）of Porana racemosa ，and to compare the contents of eight constituents . METHODS ：HPLC method was adopted. The determination was performed on Agilent TC-C 18 column with mobile phase consisted of acetonitrile- 0.1％ phosphate acid （gradient elution ） at the flow rate of 1.0 mL/min. The column temperature was 30 ℃，and the detection wavelength was set at 345 nm. The sample size was 10 μL. The contents of above constituents in stems ，twigs and leaves of P. racemosa were compared ，and the principal component analysis was carried out by Markerlynx XS software. RESULTS ：The linear range of scopolin ，scopoletin，chlorogenic acid ，cryptochlorogenic acid ， neochlorogenic acid ，isochlorogenic acid A ，isochlorogenic acid B ，and isochlorogenic acid C were 0.076 4- 7.64，0.062 8-6.28， 0.090 8-9.08，0.080 0-8.00，0.057 6-5.76，0.094 4-9.44，0.086 0-8.60，0.078 8-7.88 mg/L，respectively（all r＞0.999）. RSDs of precision，stability（24 h）and reproducibility tests were all lower than 2.0％. The average recoveries were 99.71％（RSD＝1.36％， n＝6），100.39％（RSD＝1.76％，n＝6），99.20％（RSD＝1.75％，n＝6），100.04％（RSD＝2.63％，n＝6），98.57％（RSD＝1.99％， n＝6），99.68％（RSD＝1.84％，n＝6），99.90％（RSD＝1.88％，n＝6），99.76％（RSD＝1.47％，n＝6），respectively. The average contents of above eight constituents were 9.725 3，1.286 5，7.271 3，1.347 6，0.997 7，0.710 9，0.656 3，0.364 7 mg/g in stems ； those were 0.690 3，0.411 7，4.394 3，0.639 6，0.531 3，1.392 7，0.989 1，1.129 2 mg/g in twigs ；those were 1.195 1，0.691 1， 27.952 9，6.173 4，1.405 1，0.549 7，0.288 8，0.794 2 mg/g in leaves ，respectively. The results of principal component analysis showed that different parts of P. racemosa could be divided into 3 categories. Among them ，most of the stems ofP. racemosa gathered in the first quadrant of score plot ，all the twigs gathered in the third quadrant ， and all the leaves 分m gathered in the fourth quadrant. CONCLUSIONS ：Established method is simple and reproducible ，and can be used for the determination of 8 constituents in different medicinal parts of P. racemosa. The average contents of neochlorogenic acid ，chlorogenic acid and cryptochlorogenic acid in the leaves of P. racemosa are relatively high ；the contents of isochlorogenic acid B ，isochlorogenic acid A and isochlorogenic acid C in the twigs are relatively high；the average contents of scopolin and scopoletin in the stems are also relatively high.

A new cembranoid diterpene from twigs of Euonymus alatus / 中草药

Objective To study the chemical constituents from the twigs of Euonymus alatus. Methods Compounds were isolated and purified by a combination of various chromatographic techniques, and their structures were elucidated by physiochemical property and spectral analysis. Results Three compounds were obtained from 95% ethanol extract of the twigs of E. alatus and identified as (1S,2S,7E,11R,12S)-2,11-dihydroxy-1,12-oxidocembra-4(18),7(8)-diene (1), hemerocallal A (2), and betulinic acid methyl ester (3). Conclusion Compound 1 is a new cembranoid diterpene and named euonymuditerpene A, and compounds 2 and 3 are isolated from the twigs of Euonymus alatus for the first time.

Chemical constituents from the twigs and leaves of Cryptomeria fortunei / 中成药

AIM To study the chemical constituents from the twigs and leaves of Cryptomeria fortunei Hooibrenk ex Otto et Dietr.METHODS The ethyl acetate fraction of 95％ ethanol extract from C.fortunei was isolated and purified by silica,MCI,and Sephadex LH-20 column,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Eleven compounds were isolated and identified as isopimaric acid (1),sandaracopimaric acid (2),acetylisocupressic acid (3),imbricataloic acid (4),isocupressic acid (5),pinifolic acid (6),13-epicupressic acid (7),19-acetylagathadiol (8),agatadiol (9),phytol (10),elemol (11).CONCLUSION Compounds 1-10 are identified as diterpenoids and compound 11 is identified as sesquiterpenoid;Compounds 2,3,6-11 are obtained from this plant for the first time.

Chemical constituents from twigs of Trigonostemon lutescens / 中成药

AIM To study the chemical constituents from twigs of Trigonostemon lutescens Y.T.Chang et J.Y.Liang.METHODS The ethyl acetate fraction of 95％ ethanol extract from T.lutescens twigs was isolated and purified by silica,Sephadex LH-20 and MCI column,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Ten compounds were isolated and elucidated as 3-O-acetyloleanolic acid (1),methyl 3-acetoxy-12-enly-oleanen-28-oate (2),taraxerone (3),spiciflorin (4),simiarenol (5),β-sitosterol palmitate (6),3,3',4-tri-O-methylellagic acid (7),β-sitosterol (8),stigamasterol (9),palmitic acid (10).CONCLUSION Compounds 1-6 are isolated from genus Trigonostemon for the first time,compounds 7 and 9 are first obtained from this plant.

Chemical constituents from the twigs of Morus alba / 中成药

AIM To study the chemical constituents from the twigs of Morus alba L..METHODS The 60％ ethanol extract from M.alba twigs was isolated and purified by ODS,sephadax LH-20 and semi-preparative column,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Ten compounds were isolated and identified as 4'-O-β-D-glucopyranosyl-2'-hydroxyresveratrol 3-(6-O-β-D-glucopyanosyl-β-D-glucopyranoside) (1),3-O-β-D-glucopyranosyl-2'-hydroxyresveratrol 4'-(6-O-β-D-glucopyanosyl-β-D-glucopyranoside) (2),mulberroside A (3),mulberroside E (4),mulberroside F (5),transoxyresveratrol-4'-O-β-D-glucopyranoside (6),trans-oxyresveratrol 3-O-β-D-glucopyranoside (7),cis-mulberroside A (8),3,4-dimethoxyphenyl β-D-apiofuranosyl-(1→6)-β-D-glucopyranoside (9),kelampayoside A (10).CONCLUSION Compounds 9 and 10 are isolated from genus Morus for the first time,compounds 1,2,4-7 are first isolated from this plant.

Chemical constituents from the twigs of Morus alba / 中成药

AIM To study the chemical constituents from the twigs of Morus alba L..METHODS The 60％ ethanol extract from M.alba twigs was isolated and purified by ODS,sephadax LH-20 and semi-preparative column,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Ten compounds were isolated and identified as 4'-O-β-D-glucopyranosyl-2'-hydroxyresveratrol 3-(6-O-β-D-glucopyanosyl-β-D-glucopyranoside) (1),3-O-β-D-glucopyranosyl-2'-hydroxyresveratrol 4'-(6-O-β-D-glucopyanosyl-β-D-glucopyranoside) (2),mulberroside A (3),mulberroside E (4),mulberroside F (5),transoxyresveratrol-4'-O-β-D-glucopyranoside (6),trans-oxyresveratrol 3-O-β-D-glucopyranoside (7),cis-mulberroside A (8),3,4-dimethoxyphenyl β-D-apiofuranosyl-(1→6)-β-D-glucopyranoside (9),kelampayoside A (10).CONCLUSION Compounds 9 and 10 are isolated from genus Morus for the first time,compounds 1,2,4-7 are first isolated from this plant.

Chemical constituents from twigs of Myricaria bracteata / 中草药

Objective: To investigate the chemical constituents from the twigs of Myricaria bracteata. Methods: The chemical constituents were isolated and purified by column chromatography. Their structures were identified on the basis of spectroscopic data, such as NMR, MS, and physicochemical properties. Results: Fourteen compounds were isolated from M. bracteata and identified as syringaresinol (1), (-)-lyoniresinol (2), (-)-isolariciresinol (3), N-trans-feruloyltyramine (4), N-trans-feruloyl-3-methoxytyramine (5), N-trans-feruloyl-2'-methoxytyramine (6), kaempferol-3-O-β-D-glucuronic acid methylester (7), quercetin-3-O-β-D-glucuronic acid methylester (8), quercitrin (9), rhamnocitrin (10), gallic acid (11), gallicin (12), E-caffeic acid (13), and E-ferulic acid (14). Conclusion: Compounds 1-6 and 14 are isolated from the plants of this genus for the first time. Compounds 7, 8, 12, and 13 are isolated from the twigs of M. bracteata for the first time.